Our Platforms

Our deep bioanalytical expertise allows us to be impartial in the way we utilize our array of bioanalytical platforms, tailoring our methods to meet your most rigorous assay performance demands.

Explore our extensive platform options below; be sure to click the plus buttons to the right to reveal more detailed information on each technology.

ELISA

Applications:

  • Biomarkers
  • Immunogenicity
  • Pharmacokinetics
  • Cell-Based Assays

Benefits:

  • High Precision
  • GLP & GMP
  • Wide Dynamic Range
  • Robust & Rugged
  • High Throughput
  • Improved Reliability
  • Adaptability to Automation
  • Fluorescence & Luminescence

BioAgilytix considers the ELISA method to be one of its core competencies, and has significant experience developing and optimizing ELISA kits to ensure the highest level of performance in analyte quantification. We employ a number BioTek Synergy 2 instruments to develop and run reliable, sensitive, and specific immunoassays for protein detection.

Learn More About ELISA

Download Our ELISA PDF

MSD-ECL

Applications:

  • Immunogenicity
  • Pharmacokinetics
  • Biomarkers
  • Lot Release Testing
  • Cell-Based Assays

Benefits:

  • High Sensitivity
  • Wide Dynamic Range
  • GLP & GMP
  • Robust & Rugged
  • High Throughput
  • Adaptability to Automation
  • Small Sample Volumes
  • Multiplexing

BioAgilytix uses the MSD-ECL extensively as part of our premier immunoassay facility. Although similar to a normal ELISA, MSD-ECL uses electrochemiluminesence which allows for higher sensitivity in protein detection, broader dynamic range, and lower sample volumes.

Our scientists are experts at multiplexing on the platform and our lab has been selected as the first to validate MSD’s Human Cytokine 30-Plex V-PLEX Kit. We have experience using the platform in support of non-GLP, GLP, and product release testing under GMP using samples from rodents, humans, and non-human primates.

Learn More About MSD-ECL

Download Our Meso Scale Discovery® Electrochemiluminscence PDF

Gyrolab

Applications:

  • Biomarkers
  • Pharmacokinetics
  • Lot Release Testing

Benefits:

  • High Sensitivity
  • High Precision
  • Wide Dynamic Range
  • Reduced Matrix Interference
  • GLP & GMP
  • Robust & Rugged
  • Fast Total Assay Time
  • Adaptability to Automation
  • Reduced Sample Size

In the hands of our expert assay developers, BioAgilytix’s two Gyrolab xP workstations enable the generation of quality data from small volumes of precious samples, maximizing the number of assays that can be performed.

These cutting-edge platforms use microfluidic Bioaffy CDs and include nanoliter-scale flow-through technology, automated assay workflow, and high sensitivity laser-induced flourescence (LIF) detection.

Learn More About Gyrolab

Download Our Gyrolab PDF

ProteinSimple Peggy Sue

Applications:

  • Biomarkers
  • Wide Variety of Matrices:
    • Plasma / Serum
    • Tissue Homogenates
    • Yeast and Bacteria Extracts
    • PBMCs and Other Mammalian Cell Lysates
    • Various Plant Tissues

Benefits:

  • High Throughput
  • Low Sample Volume (5 μL)
  • Capable of Improved Sensitivity Over Traditional Westerns
  • High Precision
  • Linear Dynamic Range
  • Robust & Rugged
  • Automated Western Blotting
  • Quantitative & Qualitative Assays
  • Separation Based on Size and/or Charge (IEF)
  • Total Protein Analysis

ProteinSimple assays combine capillary electrophoresis, a traditional separation technique, with an immunoassay, a well-known detection technique, allowing our team to perform quantitative Westerns with sensitivity and reproducibility unmatched by traditional Western blot methods.

Our ProteinSimple Peggy Sue platform automates Western blot assays and is a reliable, rapid alternative to traditional Western blot analysis for protein separation, identification, and quantitation. Now, quantitative Western blot analysis can be performed with high levels of sensitivity and day-to-day reproducibility.

Learn More About ProteinSimple

Learn More About Automated Western Blot (PDF)

Flow Cytometry

Applications:

  • Biomarkers
  • Cell-Based Assays
  • Immunophenotyping

Benefits:

  • Single & Multicolor
  • Drug Targeting, Potency & Molecular Internalization
  • Biomarker Expression Monitoring
  • GLP & GMP

BioAgilytix maintains two Cytek DxP8 FACSCalibur™ flow cytometers as part of its premier immunoassay facility. These robust instruments are each equipped with three solid state lasers and an improved digital acquisition system, giving us the ability to record events from eight different fluorescence parameters simultaneously.

Our scientists are skilled in a broad variety of flow cytometry applications in support of non-GLP, GLP and product release testing under GMP, with the ability to interpret as well as analyze data.

Learn More About Flow Cytometry

Download Our Flow Cytometry PDF

DELFIA

Applications:

  • Biomarkers
  • Cell-Based Assays
  • Time-Resolved Fluorescence (TRF) Assays

Benefits:

  • High Sensitivity
  • High Precision
  • Wide Dynamic Range
  • Robust & Rugged
  • Strong Stability
  • Adaptability to Automation

BioAgilytix offers immunoassay services on PerkinElmer’s DELFIA (Dissociation-Enhanced Lanthanide Fluorescent Immunoassay) platform. The system is a robust, high-performance immunodetection platform that provides a combination of benefits that make it a strong alternative to conventional ELISAs. DELFIA utilizes the unique chemical properties of lanthanide chelates in concert with time-resolved fluorescence (TRF) detection to create an assay that offers high sensitivity, wide dynamic range, superior stability, and excellent flexibility.

BioAgilytix’s immunoassay experts, in combination with the DELFIA platform, are able to qualify, develop, and run highly sensitive and stable TRF, kinase, and immunodetection assays.

Learn More About DELFIA

Download Our DELFIA PDF

LI-COR Odyssey

Applications:

  • Quantitative Western Blot Analysis
  • Two-Color Detection and Quantification
  • Infrared Imaging System
  • Protein Detection
  • In-Cell Western Assays
  • Protein Gel Imaging

Benefits:

  • High Sensitivity
  • Wide Linear Range
  • Multiplexing
  • Broad Applicability

BioAgilytix offers quantitative near-infrared fluorescence detection assays on the proven LI-COR Odyssey system. This peer-validated technology is backed by over 6,000 publications citing data from the Odyssey IR system. LI-COR enables protein detection of two targets simultaneously on the same membrane, which greatly increases accuracy of quantification and provides for more robust comparison.

Our scientific experts utilize the LI-COR Odyssey to enable highly sensitive quantitative Western assays, as well as an array of applications including protein Gel Imaging, in-cell Westerns and more.

Learn More About LI-COR

Luminex Bio-Plex

Applications:

  • Biomarkers
  • Cell-Based Assays

Benefits:

  • Sensitivity
  • Precision
  • Dynamic Range
  • Robust & Rugged
  • High Throughput
  • Adaptability to Automation
  • Large Portfolio of Multiplex Assays

BioAgilytix employs Bio-Rad’s Bio-Plex® Multiplex System to run bead-based immunoassays. Utilizing xMAP technology licensed from Luminex, the Bio-Plex multiplex system can multiplex many different assays simultaneously.

Our seasoned scientific team is skilled at leveraging the Luminex Bio-Plex multiplex system, which enables the identification of statistically relevant changes of up to 100 analytes (proteins and peptides, or nucleic acids) in a single sample volume.

Learn More About the Luminex Bio-Plex Platform

Download Our Luminex Bio-Plex PDF

Additional Platforms

  • HTRF
  • AlphaLISA
  • FIA
  • FP