An aldolase is an enzyme that performs an aldol reaction (creating an aldol) or its reverse (cleaving an aldol). The word often refers to any type of fructose-bisphosphate aldolase, but can also refer to other enzymes, such as the one that forms sialic acid.

Aldolase is an enzyme responsible for breaking down glucose products into energy, specifically converting fructose 1,6-bisphosphate into the triose phosphates dihydroxyacetone phosphate (DHAP) and glyceraldehydes 3-phosphate.