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Biomarker:

PARP (D214-cleaved)

Biological or Clinical Significance:

The Cleaved-PARP-1 (D214) pAb Antibody is a Rabbit Polyclonal antibody to PARP, PPOL, ADPRT, ADPRT1, PARP-1, PARP1, poly (ADP-ribose) polymerase 1, ARTD1. This antibody recognizes Rat, Mouse, Human antigen. The Cleaved-PARP-1 (D214) pAb Antibody has been validated for the following applications: Western Blot.

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

References:

Analyte:

PARP (D214-cleaved)

Matrix:

Human Liver Homogenates

Status:

Validated

Sensitivity-LLOQ:

5.00 ug/mL equ of PWCL

Sensitivity-ULOQ:

180 ug/mL equ of PWCL

platform

MSD-ECL

Required Sample Volume

25 µL/well

Disease State:

Oncology

MSD Panel:

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