ELISPOT for Monitoring Cellular Immune Responses
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ELISPOT for Qualitative Analysis of Cellular Immune Responses

This platform is considered among the most useful for determining cell-mediated immunity because of its sensitivity and accuracy in detecting rare antigen-specific T cells (or B cells).

BioAgilytix offers Enzyme-Linked ImmunoSpot (ELISPOT) assays at our European laboratory in Hamburg, Germany. We use ELISPOT to visualize the secretory product(s) of individual activated or responding cells to analyze the frequencies of rare antigen-specific cells within a test population—for example, single positive cells within a population of peripheral blood mononuclear cells (PBMCs).

The Benefits of ELISPOT Assays

ELISPOT offers exceptional sensitivity for the detection and enumeration of rare antigen-specific cells because of its method of capturing and secreting cytokine. ELISPOT assays employ the sandwich enzyme-linked immunosorbent assay (ELISA) technique, where either a monoclonal or polyclonal antibody specific for the chosen analyte is pre-coated onto a PVDF (polyvinylidene difluoride)-backed microplate.

Top Uses for ELISPOT:

  • Immunogenicity Testing
  • Immune Response Monitoring
  • Epitope Discovery
  • Helper T Cell Response Evaluation

Then freshly isolated, thawed or cultured cells are plated together with the antigens of interest and incubated to allow for the activation of the antigen-specific T cells and the induction of their cytokine secretion.

As the antigen-specific cells are activated, they release the cytokine, which is captured directly on the membrane surface by the immobilized antibody. The visualization of the cytokines secreted individual T- or B-clones is achieved by enzyme-conjugated or fluorescence-based substrates. The fluorescence-based method allows a multiplex analysis of antigen-specific clones. The spots are then counted with our automated ELISPOT reader system.

Platform Comparisons Guide

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Leverage the Capabilities of ELISPOT with Our Help

ELISPOT assays are able to provide qualitative information regarding the specific cytokine or other secreted immune molecule. We can also use fluorescently labeled antibodies, even in parallel via multiplexing. Our scientific team has proven their ability perform highly sensitive, high-precision monitoring of cellular immune responses using ELISPOT, and to interpret the data derived for timely results.

To speak with one of our ELISPOT experts about your scientific requirements, click here.