ELISpot for Qualitative Analysis of Cellular Immune Responses

Then freshly isolated, thawed or cultured cells are plated together with the antigens of interest and incubated to allow for the activation of the antigen-specific T cells and the induction of their cytokine secretion.

As the antigen-specific cells are activated, they release the cytokine, which is captured directly on the membrane surface by the immobilized antibody. The visualization of the cytokines secreted individual T- or B-clones is achieved by enzyme-conjugated or fluorescence-based substrates. The fluorescence-based method allows a multiplex analysis of antigen-specific clones. The spots are then counted with our automated ELISpot reader system.