Identification and Biosynthesis of an N-Glucuronide Metabolite of Camonsertib

This is the first report of a glucuronide metabolite of camonsertib formed by human hepatocyte incubations. This study reveals the structure and stability of an N-glucuronide metabolite of camonsertib using detailed elucidation by one- and two-dimensional NMR after scale-up using a novel bacterial culture approach yielding significant quantities of a purified metabolite to ensure no contribution to camonsertib during LC-MSMS samples analysis or instabilities which may contribute to detected coamonsertib concentrations during sample extraction.