Intact and Signature Peptide LCMS Bioanalysis of Three Peptide-Pegylated Lipid Conjugates from a Nanoparticle Formulation

Purpose

Traditional bioanalysis divides potential analytes between “small molecules” which are generally analyzed by LCMS, and “large molecules” which are typically analyzed by LBA (or some form of signature fragment-based LCMS). Peptide-pegylated lipid conjugates are of an intermediate size which could be measured by either approach. In this case, all three analytes were detected as intact molecules; only two had a viable tryptic site for producing a signature peptide. We developed suitable LC/MS bioanalytical methods for analyzing the three peptide PEGylated lipid conjugates from a nanoparticle formulation in plasma samples.