For Highly Specific Selection and Characterization of Biotherapeutic Candidates

Enabling label-free interaction analysis, the Biacore platform delivers data with rich content on biomolecular interactions. BioAgilytix offers the Biacore™ T200 to deliver highly specific analysis, robust data quality, and efficient time-to-results for immunogenicity studies at any phase of development.  

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BioAgilytix’s Biacore platform expertise  

From defining potential drug targets and diagnostic markers to detecting and characterizing anti-drug antibodies (ADAs) in immunogenicity studies, the BioAgilytix team has extensive experience using Biacore to perform efficient, cost-effective, high-precision bioanalysis. 

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Ideal Biacore Applications:

For high-quality characterization of biomolecular interactions 

The BioAgilytix team uses Biacore in both preclinical and clinical immunogenicity studies to analyze and monitor ADAs, including characterization and isotyping. The platform is also leveraged in the study of molecular interactions related to kinetics, specificity, and concentration, which has applications toward biological drug characterization and lead molecule selection. 

The SPR (surface plasmon resonance) on a Biacore is used for the characterization of ADAs and the measurement of therapeutic monoclonal antibodies to Fc-gamma receptors. In addition, membrane proteins such as the interaction of G protein-coupled receptors (GPCRs) with their ligands can be studied with Biacore. 

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Key Biacore Benefits:

Specificity and precision  

A key advantage of the Biacore platform is its exceptional specificity, which enables label-free interaction analysis to be performed with precision and confidence. It supports investigations that are characterized by extreme kinetics, interacting partners with low molecular weights, or physiological concentrations that would otherwise be considered beyond the limits of detection. 

Because of its specificity, this system enables interaction analysis with greater precision in understanding molecular mechanisms and structure-function relationships. In fact, The Biacore T200 offers the highest specificity of any current Biacore system, yielding exceptional data quality with increased precision of measurements that can reveal even minute differences in kinetics. 

Benefits of the Biacore platform include: 

  • High specificity 
  • High precision 
  • Label-free interaction analysis 
  • High reliability 

How the Biacore platform works  

The Biacore platform monitors molecular interactions in real time by using a non-invasive, label-free technology. This technology reacts to changes in the concentration of molecules at the sensor surface as molecules bind to or detach from the surface. 

Biacore’s detection principle is based on surface plasmon resonance (SPR) that is sensitive to changes in refractive index within about 150 nm from the sensor surface. To study the interaction between two binding partners, one partner is attached to the surface and the other is passed over the surface in a continuous flow of sample solution. The SPR response is directly proportional to the change in mass concentration close to the surface.