The LI-COR Odyssey Western blot delivers quantitative accuracy for target protein detection by using the platform’s infrared technology to match or exceed chemiluminescent detection sensitivity. Leveraging this platform’s capabilities, BioAgilytix is able to perform accurate, reproducible, quantitative Western blot assays.
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LI-COR is an infrared (IR) laser-based instrumentation that is able to detect Western blots without the need for film, a darkroom, or any chemiluminescent substrates. Using infrared fluorescent signals that are directly proportional to the amount of target protein, LI-COR Odyssey can accurately and reproducibly measure changes in protein levels via ratiometric detection. In fact, the platform is able to detect both faint and strong bands within a single image, without signal saturation.
The LI-COR imager yields very low background fluorescence, which enables detection of low-picogram targets with NIR fluorescence, as well as low abundance proteins at endogenous levels. It also enables the detection of two targets simultaneously on the same membrane, which greatly increases accuracy of quantification and provides for more robust comparison.
The LI-COR platform has broad applicability for quantitative Western blots, protein gel imaging, and in-cell Westerns, providing for quantitative analysis and a wide linear dynamic range that is not available with traditional chemiluminescent methods. The accuracy and linearity of the system’s infrared detection provides for confident analysis of the differences in protein expression, and have made LI-COR the standard for quantitative Western blots compared to film.
In addition to two-color infrared and in-gel quantitative Western blots, BioAgilytix uses our LI-COR Odyssey platform for cell-based assays, protein detection, nucleic acid detection, protein arrays, RNAi analysis, and more.
Platforms In Action:
Hw do you decide which platform to go with in support of your project? Afshin Safavi, Ph.D., of BioAgilytix offers his expert opinion in this video.
When quantitative Western blot detection or near-infrared fluorescent imaging is needed, the BioAgilytix team knows the LI-COR platform will deliver the sensitivity and linearity required to yield accurate results. LI-COR removes the need for enzymatic reactions and substrates, with highly stable signals that generate high-quality data.
Let our experts show you how we can apply LI-COR’s robust detection capabilities to your next project.