BioAgilytix employs Bio-Rad’s Luminex-based Bio-Plex Multiplex System with laser-based technology to run bead-based immunoassays. The platform gives us the ability to multiplex many different assays simultaneously, providing greater information from less sample volume and in a shorter timeframe than required for traditional immunoassay methods.
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Based on the principles of flow cytometry, the Bio-Plex platform utilizes xMAP technology licensed from Luminex to deliver its multiplexed assay capabilities. This analyzer combines advanced fluidics, optics, and digital signal processing with Luminex’s proprietary technology to perform discrete assays on the surface of color-coded beads known as ‘microspheres’.
Built around three core technologies – the fluorescently dyed microspheres, or beads, provided from Luminex; a flow cytometer with two lasers and associated optics; and a high-speed digital processor able to efficiently manage digital output – the platform employs fluorescent methods for detection, using the bead sets to identify the analytes, and detection antibodies to measure the quantity of analyte. The use of different colored beads allows for simultaneous detection of many analytes in the same sample, and laser excitation is employed to determine analyte concentration.
Luminex is ideal for bioanalytical applications that require both high plex and high throughput at the same time. It is able to yield dramatically more information from rare and / or limited samples, and decipher complex interrelationships among proteins. While traditional ELISA and other technologies excel at high throughput applications – greater than 1,000 samples per day – they lack the ability to multiplex more than five tests at a time. Alternatively, microarray technology can perform high density screening – sometimes more than 250-plex tests – but cannot yield the reproducibility needed for high throughput applications.
As a flow-based system, our Bio-Plex 200 platform reads and quantitates bead events individually, and can perform many assays on each sample. For example, a 30-plex kit can obtain up to 3,000 data points a day on up to 100 samples. BioAgilytix most frequently uses Luminex in our work with biomarkers and cell-based assays.
Platforms In Action:
Hw do you decide which platform to go with in support of your project? Afshin Safavi, Ph.D., of BioAgilytix offers his expert opinion in this video.
BioAgilytix’s scientists are well-versed in Luminex’s innovative technology, and have performed many immunoassays on the Bio-Plex platform under the guidance of non-GLP, GLP and product release testing under GMP. When you have a multiplexing need that also demands precise accuracy and reproducibility, turn to our Luminex experts for support.
To speak our scientists about your Luminex questions today and learn how can optimally support your scientific requirements.