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Expert perspectives focused on the latest bioanalytical topic & trends

Cell-Based NAb Assays: What We Need to Know

Cell-Based NAb Assays So, how do we deal with NAbs when it comes to the industrial production of biotherapeutic agents? The bioanalytical community adopts a tiered approach to tackle the problem of NAbs. Firstly, any study samples in question are first screened and confirmed for the presence of binding antibodies via an established immunoassay. If the sample tests positive for binding antibodies, it is then examined for any drug-specific neutralizing

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|cytokine diagram

The Importance of Cytokine Detection and Analysis

Cytokines are a broad class of soluble proteins, glycoproteins, and peptides that act as chemical messengers of the immune system. These small proteins are essential intercellular communicators that carry messages from one cell to another and contribute to cell growth and immune response. Cytokines include chemokines, interferons, interleukins, lymphokines and cannot cross the lipid bilayer of the cell wall to enter the cytoplasm. They have been shown to be key

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|protein color reaction test

Utilizing Bradford Assay for Protein Concentration Calculation

If your goal is to attain swift and simple protein quantification, the Bradford assay is the way to go. Let’s find out more about what makes the Bradford assay so effective and relevant over 40 years after its inception. What is Bradford Assay? The Bradford protein assay, named after its developer Marion M. Bradford, is specifically used to calculate the concentration of total protein in a sample or solution. There

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|protein structure illustration

A Beginner’s Guide to Luciferase Assays

Luciferase is an enzyme used by a variety of organisms for bioluminescence. One of the most common examples of bioluminescence exists in the biology of the firefly. Scientists have been able to wield this naturally occurring phenomena to study gene expression at the transcriptional level. Luciferase reporter assays offer rapid quantitative measurements and have a wide range of applications across various fields of cellular and molecular biology. Understanding the basic

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Strategic Bioanalytical Partnerships for the Win-Win-Win

For bioanalytical CROs, it is of critical importance to build an internal team capable of meeting their pharmaceutical and biotech customers’ drug development goals. However, many overlook the benefits of strategically aligning with external partners – those organizations that can add value to the CRO’s offerings, supplementing their solutions and services to make them more comprehensive and attractive to customers.

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Applying Cell-Based Assays, Part 2: Potency Testing for Lot Release Under GMP

In our blog discussing the use of cellular assays for neutralizing antibody (NAb) detection, we touched on the value that bioassays offer through their ability to closely mimic the effects that a biotherapeutic drug product will have on the body without the use of animals in the study. This is what makes cell-based potency assays the choice for measuring and validating the therapeutic activity of a drug product within prescribed

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Applying Cell-Based Assays, Part 1: Neutralizing Antibody (NAb) Detection

Protein-based biologic agents have the potential to induce a variety of immune responses from the body, ranging from very minor to life threatening. One such adverse immune response is generation of neutralizing antibodies (NAbs), which can bind to the biotherapeutic drug product administered and prevent it from performing its intended biologic function, in turn negatively impacting the product’s efficacy and safety profile. While standard immunoassays can be used to detect

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Assay Design for Immunogenicity Testing: Cell-Based or Ligand Binding?

Unlike small-molecule drugs, large molecule therapeutics face a high chance of detection by the immune system, and are therefore more apt to trigger alarms. The resulting immune responses can range from largely benign to life-threatening, which is why immunogenicity testing has become a critical requirement in the biologics development process. But, the development and implementation of such assays can be technically challenging, and this is causing differing industry opinions around

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