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ELISA (Enzyme-Linked Immunosorbent Assay)

For Peptide, Antibody, Hormone, and Protein Detection
Enzyme-linked immunosorbent assays (ELISA) are the most common method for ligand binding assays, and are a core competency for the BioAgilytix team. We use ELISA in both its original and automation-powered forms as a fast, reliable, and cost-effective platform for analyte quantification of specific assays used for testing of biologics as well as Cell and Gene Clinical Development Support.

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How The ELISA Method Works​

The ELISA method is designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones, and is typically performed on a 96-well plate. Using ELISA, detection is completed by assessing the conjugated enzyme activity via incubation with a substrate to produce a measurable signal.

The binding and immobilization of reagents done through ELISA makes it easy to separate bound from non-bound material during the assay, and wash away non-specifically bound materials to measure specific analytes.

The ELISA technique was originally developed in the 1970s, when it was created as a replacement for traditional radioimmunoassay methods. More recently however, the ELISA method has been paired with laboratory automation techniques to dramatically improve throughput and sensitivity. Such advancements include automated plate washing and automated readouts.

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Platform Spotlight: ELISA

Learn why ELISA is still the most used immunoassay platform for supporting large molecule bioanalysis in this featured blog from a platform expert.

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Ideal ELISA Applications: For Diverse Small And Large Molecule Study Requirements

The ELISA method is one of the most diverse available, with applications throughout health care and small and large molecule laboratory science. The platform comes in a variety of mechanisms, including direct, indirect, sandwich, competitive and ELISpot, each with its own optimal applications.

As a bioanalytical laboratory that harnesses a variety of platforms, BioAgilytix most frequently leverages ELISA in our work with biomarkers, immunogenicity, pharmacokinetics(PK), and cell-based assays. ELISA can be leveraged in a multitude of research areas, including oncology, cardiovascular, neuroscience, and gene and cell therapies. Specifically, ELISA can be used to evaluate antibodies to specific HLA haplotype expressed by allogenous cell therapy, and supports assessment of immunogenicity, both humoral and cellular response, to the transgene, vector, and lipid nanoparticle used in cell and gene therapies.

Biomarkers