ELISpot (Enzyme-Linked ImmunoSpot)

The ELISpot platform is considered among the most useful for determining cell-mediated immunity because of its sensitivity and specificity in detecting rare antigen-specific T cells (or B cells). ELISpot assays are able to provide qualitative information regarding the specific cytokine or other secreted immune molecule.

We use ELISpot to visualize the secretory product(s) of individual activated or responding cells to analyze the frequencies of rare antigen-specific cells within a test population, such as single positive cells within a population of peripheral blood mononuclear cells (PBMCs).

At BioAgilytix, we most frequently utilize ELISpot in our work with Cytotoxic T Cell Response, Helper T Cell Response, B Cell Antibody response, Epitope Discovery, immune response monitoring, epitope discovery, and helper T cell response evaluation. Specific to bioanalytical assessment of cell and gene therapies, ELISpot can be used to assess cellular immune responses against the vector. We can also use fluorescently labeled antibodies, even in parallel via multiplexing.