Flow Cytometry (FACS) for Highly Accurate Subpopulation Analysis

Flow Cytometry (FACS Analysis)

For Highly Accurate Subpopulation Analysis

Flow cytometry—commonly referred to as FACS analysis—is a method that proves very accurate and effective when used to identify and measure cellular biomarkers in complex subpopulations. BioAgilytix maintains BD FACSLyric™, BD FACSCalibur™, and Cytek DxP8 FACSCalibur flow cytometry platforms as part of our premier immunoassay facility.

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How the Flow Cytometry Platform Works

Flow cytometry platforms utilize a sophisticated array of lasers, optics, fluidics and electronic detectors to measure light scatter and/or fluorescence emission from cells, running them one at a time through a laser to quantitate physical properties of the cells, including cell size, granularity, and the amounts of target proteins. As cells pass through a laser, they absorb its light and then emit a specific color signal based on targeted fluorochromes, to determine the amount of each type of cell in the sample, or the amount of target expressed by the individual cells.

Flow cytometry allows for the high throughput, automated quantification of properties which include each particle’s relative size, granularity, internal complexity, and fluorescence intensity.

The robust flow cytometry platforms that BioAgilytix offers are each equipped with three solid state lasers and an improved digital acquisition system, giving us the ability to record events from eight different fluorescence parameters simultaneously.

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Ideal Applications for FACS Analysis: Detection and Measurement in Complex Subpopulations

Flow cytometry is a method that proves very accurate and effective when used to identify and measure cellular biomarkers in complex subpopulations. Flow cytometry is used in molecular biology, immunology, pathology, and plant biology. It is also a vital laboratory process used in transplantation, oncology, hematology, genetics and prenatal diagnosis.

BioAgilytix typically uses its premier flow cytometry platforms for services involving biomarkers, immunogenicity, cell-based assays, and immunophenotyping. We find this platform especially valuable for high-throughput, high-accuracy cell counting, and well as for applications involving biomarker detection and protein engineering.





cell-based assays

Cell-Based Assays

Key Flow Cytometry Benefits: Rapid Analysis on a Cell-by-Cell Basis

A key advantage of flow cytometry is its ability to analyze and measure cells individually. This is particularly beneficial when studying heterogeneous populations of cells, as it can be used to rapidly analyze subpopulations in just a few minutes, producing very detailed data on count, properties, and classification on a cell-by-cell basis.

As such, flow cytometry has the benefit of always highlighting non-uniformity in a uniform cell population. Cells of interest in a sample can then be sorted out for further testing as needed. Flow cytometry platforms also remove debris and dead cells from final data output, ensuring high accuracy.

flow cytometry facs analysis report

Top Benefits of FACS Analysis:

  • Single and Multicolor
  • Biomarker Expression Monitoring
  • Drug Targeting, Potency & Molecular Internalization
  • Strong Accuracy
flow cytometry

Platforms In Action:

Recommendations on bioanalytical method stability implications of co-administered and co-formulated drugs by Global CRO Council for Bioanalysis (GCC)

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Bioagilytix’s Flow Cytometry Expertise

BioAgilytix’s scientists are skilled in a broad variety of flow cytometry applications in support of non-GLP, GLP and product release testing under GMP, with proven ability to successfully interpret and analyze the data generated from our Cytek DxP8 FACSCalibur™ flow cytometers.

When you have a need for accurate and efficient analysis with flow cytometry, turn to the veteran experts on the BioAgilytix team.

scientist performing facs analysis

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