Flow cytometry—commonly referred to as FACS analysis — is an accurate and effective method when used to identify and measure cellular biomarkers in complex subpopulations. BioAgilytix utilizes the Beckman Coulter CytoFLEX LX, BD FACSLyric™, and BD FACSCanto™ II flow cytometry platforms as part of our premier immunoassay facilities.
Learn More About Flow Cytometry:
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Flow cytometry platforms utilize a sophisticated array of lasers, optics, fluidics and electronic detectors to measure light scatter and/or fluorescence emission from cells, running them one at a time through a laser to quantitate physical properties of the cells, including cell size, granularity, and the amounts of target proteins. As cells pass through a laser, they absorb its light and then emit a specific color signal based on targeted fluorochromes, to determine the amount of each type of cell in the sample, or the amount of target expressed by the individual cells.
Flow cytometry allows for accurate and precise analyses of cell surface and intracellular molecules to better characterize and define cell types in a mixed or purified sample. It enables the high throughput, automated quantification of properties which include each particle’s relative size, granularity, internal complexity, and fluorescence intensity.
Our passion for the science of small and large molecule bioanalysis drives our ability to deliver premium, tailored services for biomarker, immunogenicity, pharmacokinetics (PK), and cell-based assays.
BioAgilytix typically uses its premier flow cytometry platforms for services involving immunogenicity, cell-based assays, and intricate immunophenotyping, oncological, and disease-associated biomarker analysis and monitoring. We also leverage this platform for DNA and RNA analysis, receptor & target occupancy assays, intracellular and surface marker analysis and/or phenotyping, whole cell and receptor binding assays, and in vitro toxicology, antibody screening, and rare event analysis, such as in dendritic cells and stem/progenitor cells.
Biomarkers
Immunogenicity
Cell-Based Assays
The use of multi-color flow cytometry has a host of applications in basic research, drug discovery and the clinical laboratory. A key advantage of flow cytometry is its ability to analyze and measure cells individually. This is particularly beneficial when studying heterogeneous populations of cells, as it can be used to rapidly analyze subpopulations in just a few minutes, producing very detailed data on count, properties, and classification on a cell-by-cell basis.
We find this platform especially valuable for high-throughput, high-accuracy cell counting, and well as for applications involving biomarker detection and protein engineering.
Flow cytometry enables in-depth analysis of processes such as:
Platforms In Action:
BioAgilytix’s scientists are skilled in a broad variety of flow cytometry applications in support of non-GLP, GLP and product release testing under GMP, with proven ability to successfully interpret and analyze the data generated from our Beckman Coulter CytoFLEX LX, BD FACSLyric™, and BD FACSCanto™ II flow cytometers.
When you have a need for accurate and efficient analysis with flow cytometry, turn to the veteran experts on the BioAgilytix team.
See how our premier bioanalytical services support all phases of biomarker, immunogenicity, PK, and cell-based assay studies.
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