Flow cytometry—commonly referred to as FACS analysis—is a method that proves very accurate and effective when used to identify and measure cellular biomarkers in complex subpopulations. BioAgilytix maintains BD FACSLyric™, BD FACSCalibur™, and Cytek DxP8 FACSCalibur flow cytometry platforms as part of our premier immunoassay facility.
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Flow cytometry platforms utilize a sophisticated array of lasers, optics, fluidics and electronic detectors to measure light scatter and/or fluorescence emission from cells, running them one at a time through a laser to quantitate physical properties of the cells, including cell size, granularity, and the amounts of target proteins. As cells pass through a laser, they absorb its light and then emit a specific color signal based on targeted fluorochromes, to determine the amount of each type of cell in the sample, or the amount of target expressed by the individual cells.
Flow cytometry allows for the high throughput, automated quantification of properties which include each particle’s relative size, granularity, internal complexity, and fluorescence intensity.
The robust flow cytometry platforms that BioAgilytix offers are each equipped with three solid state lasers and an improved digital acquisition system, giving us the ability to record events from eight different fluorescence parameters simultaneously.
Use our Platform Comparison Guide to evaluate the optimal method and technology for your assay, based on its unique characteristics and performance requirements.
Flow cytometry is a method that proves very accurate and effective when used to identify and measure cellular biomarkers in complex subpopulations. Flow cytometry is used in molecular biology, immunology, pathology, and plant biology. It is also a vital laboratory process used in transplantation, oncology, hematology, genetics and prenatal diagnosis.
BioAgilytix typically uses its premier flow cytometry platforms for services involving biomarkers, immunogenicity, cell-based assays, and immunophenotyping. We find this platform especially valuable for high-throughput, high-accuracy cell counting, and well as for applications involving biomarker detection and protein engineering.
Biomarkers
Immunogenicity
Cell-Based Assays
A key advantage of flow cytometry is its ability to analyze and measure cells individually. This is particularly beneficial when studying heterogeneous populations of cells, as it can be used to rapidly analyze subpopulations in just a few minutes, producing very detailed data on count, properties, and classification on a cell-by-cell basis.
As such, flow cytometry has the benefit of always highlighting non-uniformity in a uniform cell population. Cells of interest in a sample can then be sorted out for further testing as needed. Flow cytometry platforms also remove debris and dead cells from final data output, ensuring high accuracy.
Top Benefits of FACS Analysis:
Platforms In Action:
BioAgilytix’s scientists are skilled in a broad variety of flow cytometry applications in support of non-GLP, GLP and product release testing under GMP, with proven ability to successfully interpret and analyze the data generated from our Cytek DxP8 FACSCalibur™ flow cytometers.
When you have a need for accurate and efficient analysis with flow cytometry, turn to the veteran experts on the BioAgilytix team.
See how our premier bioanalytical services support all phases of biomarker, immunogenicity, PK, and cell-based assay studies.
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