Flow Cytometry (FACS) for Highly Accurate Subpopulation Analysis

Flow Cytometry (FACS Analysis)

For Highly Accurate Subpopulation Analysis

Flow cytometry—commonly referred to as FACS analysis — is an accurate and effective method when used to identify and measure cellular biomarkers in complex subpopulations. BioAgilytix utilizes the Beckman Coulter CytoFLEX LX, BD FACSLyric™, and BD FACSCanto™ II flow cytometry platforms as part of our premier immunoassay facilities.

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How the Flow Cytometry Platform Works

Flow cytometry platforms utilize a sophisticated array of lasers, optics, fluidics and electronic detectors to measure light scatter and/or fluorescence emission from cells, running them one at a time through a laser to quantitate physical properties of the cells, including cell size, granularity, and the amounts of target proteins. As cells pass through a laser, they absorb its light and then emit a specific color signal based on targeted fluorochromes, to determine the amount of each type of cell in the sample, or the amount of target expressed by the individual cells.

Flow cytometry allows for accurate and precise analyses of cell surface and intracellular molecules to better characterize and define cell types in a mixed or purified sample. It enables the high throughput, automated quantification of properties which include each particle’s relative size, granularity, internal complexity, and fluorescence intensity.

The robust flow cytometry platforms that BioAgilytix offers are each equipped with three solid state lasers and an improved digital acquisition system, giving us the ability to record events from eight different fluorescence parameters simultaneously.

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Our passion for the science of small and large molecule bioanalysis drives our ability to deliver premium, tailored services for biomarker, immunogenicity, pharmacokinetics (PK), and cell-based assays.

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Ideal Applications for FACS Analysis: Detection and Measurement in Complex Subpopulations

Flow cytometry is a method that proves very accurate and effective when used to identify and measure cellular biomarkers in complex subpopulations. It is an incomparable assay design approach to better analyze multiple parameters, with high-throughput capability designed to maximize efficiency while preserving accuracy and precision. It is also a vital laboratory process used in transplantation, oncology, hematology, genetics and prenatal diagnosis.

BioAgilytix typically uses its premier flow cytometry platforms for services involving immunogenicity, cell-based assays, and intricate immunophenotyping, oncological, and disease-associated biomarker analysis and monitoring. We also leverage this platform for DNA and RNA analysis, receptor & target occupancy assays, intracellular and surface marker analysis and/or phenotyping, whole cell and receptor binding assays, and in vitro toxicology, antibody screening, and rare event analysis, such as in dendritic cells and stem/progenitor cells.





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Cell-Based Assays

Key Flow Cytometry Benefits: Rapid Analysis on a Cell-by-Cell Basis

The use of multi-color flow cytometry has a host of applications in basic research, drug discovery and the clinical laboratory. A key advantage of flow cytometry is its ability to analyze and measure cells individually. This is particularly beneficial when studying heterogeneous populations of cells, as it can be used to rapidly analyze subpopulations in just a few minutes, producing very detailed data on count, properties, and classification on a cell-by-cell basis.

As such, flow cytometry has the benefit of always highlighting non-uniformity in a uniform cell population. Cells of interest in a sample can then be sorted out for further testing as needed. Flow cytometry platforms also remove debris and dead cells from final data output, ensuring high accuracy.

We find this platform especially valuable for high-throughput, high-accuracy cell counting, and well as for applications involving biomarker detection and protein engineering.

flow cytometry facs analysis report

Flow cytometry enables in-depth analysis of processes such as:

  • Cell cycle regulation
  • Cell proliferation
  • Ploidy determination
  • Intracellular degradation (autophagy)
  • Apoptosis
  • Senescence
  • Necrosis
  • Oncogenesis
  • Functional studies within phenotypically defined populations
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Platforms In Action:

Recommendations on bioanalytical method stability implications of co-administered and co-formulated drugs by Global CRO Council for Bioanalysis (GCC)

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Bioagilytix’s Flow Cytometry Expertise

BioAgilytix’s scientists are skilled in a broad variety of flow cytometry applications in support of non-GLP, GLP and product release testing under GMP, with proven ability to successfully interpret and analyze the data generated from our Beckman Coulter CytoFLEX LX, BD FACSLyric™, and BD FACSCanto™ II flow cytometers.

When you have a need for accurate and efficient analysis with flow cytometry, turn to the veteran experts on the BioAgilytix team.

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