Flow Cytometry

For Highly Accurate Subpopulation Analysis

Flow cytometry is an accurate and effective method when used to identify and measure cellular biomarkers in complex subpopulations. BioAgilytix utilizes the Beckman Coulter CytoFLEX LX, BD FACSLyric™, and BD FACSCanto™ II flow cytometry platforms as part of our premier immunoassay facilities.

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HOW THE FLOW CYTOMETRY PLATFORM WORKS

Flow cytometry platforms utilize a sophisticated array of lasers, optics, fluidics and electronic detectors to measure light scatter and/or fluorescence emission from cells, running them one at a time through a laser to quantitate physical properties of the cells, including cell size, granularity, and the amounts of target proteins. As cells pass through a laser, they absorb its light and then emit a specific color signal based on targeted fluorochromes, to determine the amount of each type of cell in the sample, or the amount of target expressed by the individual cells.

Flow cytometry allows for accurate and precise analyses of cell surface and intracellular molecules to better characterize and define cell types in a mixed or purified sample. It enables the high throughput, automated quantification of properties which include each particle’s relative size, granularity, internal complexity, and fluorescence intensity.

Why BioAgilytix for Small and Large Molecule Bioanalysis?

Our passion for the science of small and large molecule bioanalysis drives our ability to deliver premium, tailored services for biomarker, immunogenicity, pharmacokinetics (PK), and cell-based assays.

IDEAL APPLICATIONS FOR Flow Cytometry ANALYSIS: DETECTION AND MEASUREMENT IN COMPLEX SUBPOPULATIONS

BioAgilytix typically uses its premier flow cytometry platforms for services involving immunogenicitycell-based assays, and intricate immunophenotyping, oncological, and disease-associated biomarker analysis and monitoring. We also leverage this platform for DNA and RNA analysis, receptor & target occupancy assays, intracellular and surface marker analysis and/or phenotyping, whole cell and receptor binding assays, and in vitro toxicology, antibody screening, and rare event analysis, such as in dendritic cells and stem/progenitor cells.