Luminex Bio-Plex® Bead-Based Immunoassay Multiplex System

Luminex Platforms

For Multiplexing of Bead-Based Immunoassays

BioAgilytix employs Bio-Rad’s Luminex-based Bio-Plex Multiplex System with laser-based technology and Luminex FLEXMAP 3D® systems to run bead-based immunoassays. These platforms give us the ability to multiplex many different assays simultaneously, providing greater information from less sample volume and in a shorter timeframe than required for traditional immunoassay methods.

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luminex flexmap 3d

How the Luminex Platforms Work

Based on the principles of flow cytometry, the Bio-Plex platform utilizes xMAP technology licensed from Luminex to deliver its multiplexed assay capabilities. This analyzer combines advanced fluidics, optics, and digital signal processing with Luminex’s proprietary technology to perform discrete assays on the surface of color-coded beads known as ‘microspheres’.

Built around three core technologies – the fluorescently dyed microspheres, or beads, provided from Luminex; a flow cytometer with two lasers and associated optics; and a high-speed digital processor able to efficiently manage digital output – the platform employs fluorescent methods for detection, using the bead sets to identify the analytes, and detection antibodies to measure the quantity of analyte. The use of different colored beads allows for simultaneous detection of many analytes in the same sample, and laser excitation is employed to determine analyte concentration.

The main advantages of the Luminex FLEXMAP 3D system are the ability to multiplex up to 500 analytes with minimal sample volume, and automation to support high-throughput bioassay applications.

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Our passion for the science of small and large molecule bioanalysis drives our ability to deliver premium, tailored services for biomarker, immunogenicity, pharmacokinetics (PK), and cell-based assays.

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Ideal Luminex Applications: For Simultaneously High Plex and Throughput

Luminex is ideal for bioanalytical applications that require both high plex and high throughput at the same time. It is able to yield dramatically more information from rare and / or limited samples, and decipher complex interrelationships among proteins. While traditional ELISA and other technologies excel at high throughput applications – greater than 1,000 samples per day – they lack the ability to multiplex more than five tests at a time. Alternatively, microarray technology can perform high density screening – sometimes more than 250-plex tests – but cannot yield the reproducibility needed for high throughput applications.

As a flow-based system, our Luminex platforms read and quantitate bead events individually, and can perform many assays on each sample. For example, a 30-plex kit can obtain up to 3,000 data points a day on up to 100 samples. BioAgilytix most frequently uses Luminex in our work with biomarkers and cell-based assays. Our team has optimized and validated several cytokine, chemokine, growth factor, and biomarker assays ranging from known vendor protein panels (i.e., pro- or anti-inflammatory panels) to completely customized panels. We can also perform non-regulated analysis of antibodies to specific HLA haplotype expressed by allogenous cell therapy using Luminex.



cell-based assays

Cell-Based Assays

Key Luminex Benefits: Multiplexing and High Speed and Throughput

The multiplexing functionality enabled by Luminex’s technology reduces time, labor, and costs over traditional methods such as ELISA or Western blot, and is particularly advantageous for small sampling volumes. The dual laser-based reader is able to deliver robust data from proteins in a very small volume. Throughput is also maximized, as each Luminex microsphere serves as an individual test, so that a large number of assays can be performed and analyzed at once.

Luminex also offers strong versatility in that the single xMAP technology-based system is able to perform a multitude of assay types, from nucleic acids and antigen-antibody binding, to enzyme, receptor-ligand, and other protein interactions. Reproducibility is optimized as well, since the high-volume processing of Luminex microspheres within a single lot allows for assay standardization that solid-phased planar arrays cannot provide.

luminex bioplex analysis report

Top Benefits of Luminex Bio-Plex:

  • Multiplexing
  • High Speed and Throughput
  • Small Sample Volumes
  • Versatility and Flexibility
  • High Accuracy
  • Reproducibility
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Platforms In Action:

Virtual Podium: An Analytical Approach to Platform Comparison

Hw do you decide which platform to go with in support of your project? Afshin Safavi, Ph.D., of BioAgilytix offers his expert opinion in this video.

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Bioagilytix’s Luminex Platform Expertise

BioAgilytix’s scientists are well-versed in Luminex’s innovative technology, and have performed many immunoassays on the Bio-Plex platform under the guidance of non-GLP, GLP and product release testing under GMP. We also operate and maintain two Luminex FLEXMAP 3D systems with xPonent software in our Boston, MA laboratory, and both are 21 CFR Part 11 compliant. We have multiple data analysis software options, and pride ourselves on being versatile with data analysis. We are well versed in the Bio-Plex Manager analytical software, which is designed to remedy the challenges associated with xMAP instruments (FLEXMAP 3D) and analyzing its multiplex data.

When you have a multiplexing need that also demands precise accuracy and reproducibility, turn to our Luminex experts for support.

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