Meso Scale Discovery® Electrochemiluminescence to Support Your Multiplexing Needs

Meso Scale Discovery®
Electrochemiluminescence (MSD-ECL)

Ultra-Sensitive Detection to Support Your Multiplexing Needs

BioAgilytix leverages the Meso Scale Discovery Electrochemiluminescence (MSD-ECL) platform and its plate-based electrochemical luminescence technology to provide reliable, high quality data for a diverse variety of biological assay used for the development of biologics and cell and gene therapies. MSD is particularly well-suited for those assays characterized by high sensitivity and high tolerance to circulating antigen.

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msd ecl platform analytical device

How the Meso Scale Discovery Platform (MSD-ECL) Works

The MSD-ECL platform uses electrochemiluminescent labels that are conjugated to detection antibodies. These labels generate light when stimulated by electricity in the appropriate chemical environment, which can then be used to measure key proteins and molecules.

The detection process is initiated at electrodes located in the bottom of the platform’s microplates, and only labels near the electrode are excited and detected. Electricity is applied to the plate electrodes, leading to light emission by SULFO-TAG labels, which are electrochemiluminescent labels that allow for ultra-sensitive detection. Light intensity is then measured to quantify analytes in the sample. The MSD-ECL platform employs buffers with high concentrations of Tripropylamine as a catalyst for a dual redox reaction with Ruthenium, emitting light at 620 nm.

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Why BioAgilytix for Large Molecule Bioanalysis?

Our passion for the science of large molecule bioanalysis drives our ability to deliver premium, tailored services for biomarker, immunogenicity, pharmacokinetics (PK), and cell-based assays.

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Ideal MSD Applications: For Multiplexing and Complex Matrices

The MSD-ECL platform offers great flexibility with stable, non-radioactive, and easily conjugated labels. Because of these factors, it makes a strong alternative to conventional colorimetric methods like ELISA when higher sensitivity and multiplexing are critical. We have also found MSD assays are optimal for complex matrices as they typically yield low interference from components like serum or plasma. MSD is particularly advantageous in that its bridging formats are not specific for species or antibody subclass.

BioAgilytix’s scientists most frequently utilize Meso Scale Discovery (MSD-ECL) in our work with biomarkers, immunogenicity, pharmacokinetics (PK), cell-based assays, and lot release testing, as well as for the bioanalysis of cell and gene therapies – which could include evaluation of antibodies to specific HLA haplotype expressed by allogenous cell therapy and immunogenicity assessments for the vector, transgene, and lipid nanoparticle used in these compounds. We are able to run MSD assays in support of non-GLP, GLP, and product release testing under GMP.

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cell-based assays

Cell-Based Assays

Key MSD-ECL Benefits: High Sensitivity and Broad Dynamic Range

Although similar in many respects to the traditional ELISA method, the Meso Scale Discovery platform (MSD-ECL) uses non-radioactive electrochemiluminescent labels for ultra-sensitive detection, yielding higher sensitivity and broader dynamic range, all at lower sample volumes.

Electrochemiluminescence offers significant advantages over traditional ELISA assays including low background, signal amplification, improved sensitivity, and dynamic range. In a multiplex array, the MSD platform facilitates the detection of up to 10 analytes from a single sample. The MSD catalog includes a large selection of single and multiplex kits for profiling biomarkers, cell signaling pathways as well as other applications.

Additionally, this immunoassay method decouples the stimulation – electricity – from the signal – light – so that only labels near the electrode surface are detected, ensuring low background. This gives our scientists the ability to quickly derive quality data in a variety of sample types, including cell supernatant, serum, plasma, and whole blood.

msd ecl analysis report

Top Benefits of MSD Assays:

  • High Sensitivity
  • Broad Dynamic Range
  • Robust & Rugged
  • High Throughput
  • Adaptability to Automation
  • Small Sample Volumes
  • Multiplexing

MSD In Action:

Evaluation of MSD-ECL Multiplex Immunoassay for the Determination of Cardiac Toxicity Biomarkers in Rat Serum

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Bioagilytix’s MSD-ECL Platform Expertise

BioAgilytix’s scientists are proven experts at multiplexing on the Meso Scale Discovery platform (MSD-ECL), and our Durham, NC lab was selected as the first to validate MSD’s Human Cytokine 30-Plex V-PLEX Kit. Our scientific team in Boston, MA also has significant experience in leveraging MSD for receptor occupancy assays such as free uninhibited Btk target site occupancy in human B cell lysates.

We currently operate Imager 6000, Sector S 600, and QuickPlex SQ 120 systems from MSD, all of which are GLP-compliant, within our labs in Massachusetts, North Carolina, and Germany.

We have particular expertise using MSD-ECL for complex matrices, and have had proven success meeting the demands of even the most challenging bioanalytical assays.

btk target occupancy assay using msd

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