Quantitative Real-Time PCR (qPCR)

Real-Time PCR / Quantitative PCR (qPCR)

A Powerful, High-Throughput Tool for Your Molecular Testing Needs

BioAgilytix leverages real-time PCR (also known as quantitative PCR or qPCR) to deliver genomics services supporting the preclinical and clinical phases of drug development. Our molecular suite is equipped with the latest generation of PCR equipment, including Applied Biosystems ViiA 7 with both 96- and 384-well fast-cycle options, as well as TaqMan Array Cards.

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Ideal PCR Applications: For High-Quality Genomics Results at Any Phase of Development

BioAgilytix’s scientists utilize qPCR assays in our work with biomarkers, pharmacokinetics/pharmacodynamics (PK/PD), and for the bioanalysis of cell and gene therapies, delivering premier genomics services to support sponsors’ molecular testing needs.

Our molecular suite is custom designed with separate facilities for automated DNA and RNA extraction, from a variety of sample types. We record the nucleic acid yield and purity in all cases to ensure the highest quality genomics results. Our test menu includes custom, real-time qPCR, gene expression analysis using TaqMan assays, SNP genotyping analysis and copy number variation, and mutation detection using TaqMan and microRNA analysis.

real time pcr biomarkers



pcr for gene therapy

Gene Therapy

pcr for cell therapy

Cell Therapy

bioanalysis for car t therapies

qpcr for car-t cell therapy bioanalysis

Supporting CAR-T Therapies: Innovation to Overcome Bioanalytical Challenges

There are many bioanalytical challenges that accompany development of cell therapies such as CAR-T therapies – which is why we are focused on continually refining and innovating our methods to address those complexities. Learn how we leverage platforms like qPCR, flow cytometry, and ELISpot to meet the specificity, selectivity, and sensitivity requirements of each unique study.

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real time pcr platform

How Real-Time PCR (qPCR) Works

Real-time polymerase chain reaction (rtPCR or qPCR) is an essential method for detecting nucleic acids and has a wide range of clinical and research applications. It monitors the progression of DNA amplification by measuring the signal of fluorescent molecules (chromaphores) activated during the PCR process, which is implemented to multiply DNA.

Modern qPCR instrumentation allows for the simultaneous measurement of multiple DNA amplification reactions in a single reaction vessel, making it possible to process a large number of samples simultaneously and also to perform a large number of assays per sample. This enables reaction volumes at nanoliter levels, creating substantial savings in the amount of reagents and sample needed to perform testing.

BioAgilytix’s qPCR Platform Expertise

Our scientific team offers a breadth and depth of expertise in the development, optimization, and validation of complex molecular assays using qPCR, working in collaboration with your team to ensure that your project is a success. We are able to leverage our Applied Biosystems ViiA 7 system, which provides enhanced, high-resolution fluorescence detection, for precise quantification and mulitplexing flexibility.

Learn how we can support your unique qPCR assay requirements with our PCR platform today.

cro with pcr qpcr platform

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